Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: Actin (smooth muscle) , REAL650 , 50 , 130-123-363 , FITC , Miltenyi Biotec.

Techniques: Imaging

Journal: Scientific Reports

Article Title: The antifibrotic effect of Vildagliptin and Diaminodiphenyl Sulfone in murine schistosomiasis mansoni

doi: 10.1038/s41598-025-91955-4

Figure Lengend Snippet: Effect of Vilda, DDS, and Vilda/DDS on α-SMA and MMP-9 as fibrotic markers on S. mansoni- infected mice. ( a ) Photomicrographs of immunohistochemical staining (IHC) of hepatic tissues with alpha-smooth muscle actin (α-SMA) (× 200, scale bar = 100µm) as follows: liver sections of normal control and normal treated groups (Control, Vilda, DDS, and Vilda/DDS) marked scarce hepatocytes with positive cytoplasmic reactivity to α-SMA antibody (arrow). Liver sections from (Infected to Inf + PZQ) highlighted positive expression of α-SMA along ova of Schistosoma (arrowhead), fibroblast encircling granuloma (circle), inflammatory cells with nuclear expression (cube), and surrounding hepatocytes with cytoplasmic reactivity (arrow). The expressions were graded as intense in the Infected group, high in Inf + DDS group , moderately high in Inf + PZQ group , moderately low in Inf + Vilda/DDS group , and few in Inf + Vilda group. ( b ) Quantitative analysis of hepatic α-SMA IHC expression; (c) Hepatic level of matrix metallopeptidase-9 (MMP-9) as a fibrotic marker . Data are presented as mean ± SEM, (n = 6) and analyzed by one-way ANOVA followed by Tukey multiple comparisons. a Significantly different from the normal untreated group (Control), b Significantly different from the S. mansoni -infected untreated group (infected), c Significantly different from Inf + PZQ group, and d Significantly different from Inf + Vilda/DDS group, at P < 0.05. Inf + PZO, infected mice treated with paraziquantel; Inf + Vilda/DDS, infected mice treated with a combination of vildagliptin and diaminodiphenyl sulphone; Inf + Vilda, infected mice treated with vildagliptin; Inf + DDS, infected mice treated with diaminodiphenyl sulphone. All treatments were administered for 14 consecutive days, except PZQ for two consecutive days.

Article Snippet: Regarding immunohistochemical examination, thick deparaffinized liver Sects. (3–5 μm) were evaluated for the immunohistochemical reactivity of Anti-Rabbit α-SMA polyclonal Antibody (Cat#E-AB-34268, Elabscience, USA) and Anti-TGF-beta-1 polyclonal Antibody (Cat#ARG56429, Ari Gobio), following the manufacturer’s procedure.

Techniques: Infection, Immunohistochemical staining, Staining, Control, Expressing, Marker

Journal: Scientific reports

Article Title: An inspired microenvironment of cell replicas to induce stem cells into keratocyte-like dendritic cells for corneal regeneration.

doi: 10.1038/s41598-023-42359-9

Figure Lengend Snippet: Figure 2. Keratocytes and ADSCs characterization. (a) Light microscopy photographs of cultured keratocytes with dendritic morphology. (b) The gene expression profile demonstrated higher expression of positive keratocyte markers, including Lumican (LUM), Keratocan (KERA), ALDH3A1, and CD34 and minimal expression of myofibroblast marker (ACTA2) in keratocytes and ADSCs. (c,d) Immunofluorescence images of human keratocytes cultured for Keratocan (green)/lumican (red)/nuclei (DAPI, blue), and α-SMA (green)/ nuclei (DAPI, blue). (e) Representative of average protein levels. (f) Light inverted microscopy photographs of ADSCs with spindle shape morphology. (g) Flow cytometry results showing up-expression of CD105, CD73, CD90, and down-expression of CD45 in hADSCs.

Article Snippet: Cell 4 Vol:. (1234567890) Scientific Reports | (2023) 13:15012 | https://doi.org/10.1038/s41598-023-42359-9 nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI) (1 mg/ mL; Santa Cruz Biotechnology, USA) for 5 min. To examine myofibroblast phenotype, the cells after fixation and permeabilization were diluted by rabbit polyclonal antibody to α-SMA (1:100 dilution, Biorbyt, UK) overnight at 4 °C.

Techniques: Light Microscopy, Cell Culture, Gene Expression, Expressing, Marker, Immunofluorescence, Inverted Microscopy, Flow Cytometry

Journal: Scientific reports

Article Title: An inspired microenvironment of cell replicas to induce stem cells into keratocyte-like dendritic cells for corneal regeneration.

doi: 10.1038/s41598-023-42359-9

Figure Lengend Snippet: Figure 8. Immunocytochemistry of seeded cells for protein expression after 2 and 3 weeks’ cultivation on patterned PDMS (KBM), patterned PDMS (DMEM/F12) and plain PDMS (KBM) at 40 X magnification. (a,d) Double staining for lumican (red) and keratocan (green) in ADSCs cultivated on patterned PDMS (KBM), patterned PDMS (DMEM/F12), and plain PDMS (KBM) after 14 and 21-days. (b,e) Expression of α-SMA protein (green) in the ADSCs on patterned PDMS (KBM and DMEM/F12), and plain PDMS (KBM). (c,f) Statistical analyses for cultured ADSCs on the imprinted substrates (KBM and DMEM/F12), compared to plain PDMS (KBM) at day 14 and 21, respectively. Data are mean ± SD. ***p < 0.001. ns nonsignificant.

Article Snippet: Cell 4 Vol:. (1234567890) Scientific Reports | (2023) 13:15012 | https://doi.org/10.1038/s41598-023-42359-9 nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI) (1 mg/ mL; Santa Cruz Biotechnology, USA) for 5 min. To examine myofibroblast phenotype, the cells after fixation and permeabilization were diluted by rabbit polyclonal antibody to α-SMA (1:100 dilution, Biorbyt, UK) overnight at 4 °C.

Techniques: Immunocytochemistry, Expressing, Double Staining, Cell Culture